Proximal tubules eliminate endocytosed gold nanoparticles through an organelle-extrusion-mediated self-renewal mechanism

Hall, J.E. & Hall, M. E. Guyton and Hall Textbook of Medical Physiology (Elsevier Health Sciences, 2010).

Du, B. et al. Glomerular barrier behaves as an atomically precise bandpass filter in a sub-nanometre regime. Nat. Nanotechnol. 12, 1096–1102 (2017).

Article  CAS  Google Scholar 

Zhuo, J. L. & Li, X. C. Proximal nephron. Compr. Physiol. 3, 1079–1123 (2013).

Article  Google Scholar 

Gudehithlu, K. P., Pegoraro, A. A., Dunea, G., Arruda, J. A. L. & Singh, A. K. Degradation of albumin by the renal proximal tubule cells and the subsequent fate of its fragments. Kidney Int. 65, 2113–2122 (2004).

Article  CAS  Google Scholar 

Sancey, L. et al. Long-term in vivo clearance of gadolinium-based AGuIX nanoparticles and their biocompatibility after systemic injection. ACS Nano 9, 2477–2488 (2015).

Article  CAS  Google Scholar 

Tenten, V. et al. Albumin is recycled from the primary urine by tubular transcytosis. J. Am. Soc. Nephrol. 24, 1966–1980 (2013).

Article  CAS  Google Scholar 

Du, B., Yu, M. & Zheng, J. Transport and interactions of nanoparticles in the kidneys. Nat. Rev. Mater. 3, 358–374 (2018).

Article  Google Scholar 

Oh, N. & Park, J.-H. Endocytosis and exocytosis of nanoparticles in mammalian cells. Int. J. Nanomed. 9, 51–63 (2014).

Google Scholar 

Chithrani, B. D. & Chan, W. C. W. Elucidating the mechanism of cellular uptake and removal of protein-coated gold nanoparticles of different sizes and shapes. Nano Lett. 7, 1542–1550 (2007).

Article  CAS  Google Scholar 

Balfourier, A. et al. Unexpected intracellular biodegradation and recrystallization of gold nanoparticles. Proc. Natl Acad. Sci. USA 117, 103–113 (2019).

Article  Google Scholar 

Kim, C. et al. Regulating exocytosis of nanoparticles via host–guest chemistry. Org. Biomol. Chem. 13, 2474–2479 (2015).

Article  CAS  Google Scholar 

Ho, L. W. C., Yin, B., Dai, G. & Choi, C. H. J. Effect of surface modification with hydrocarbyl groups on the exocytosis of nanoparticles. Biochemistry 60, 1019–1030 (2021).

Article  CAS  Google Scholar 

Ho, L. W. C. et al. Mammalian cells exocytose alkylated gold nanoparticles via extracellular vesicles. ACS Nano 16, 2032–2045 (2022).

Article  CAS  Google Scholar 

Tang, S., Huang, Y. & Zheng, J. Salivary excretion of renal-clearable silver nanoparticles. Angew. Chem. Int. Ed. 59, 19894–19898 (2020).

Article  CAS  Google Scholar 

Soo Choi, H. et al. Renal clearance of quantum dots. Nat. Biotechnol. 25, 1165–1170 (2007).

Article  Google Scholar 

Christensen, E. I., Rennke, H. G. & Carone, F. A. Renal tubular uptake of protein: effect of molecular charge. Am. J. Physiol. Ren. Physiol. 244, F436–F441 (1983).

Article  CAS  Google Scholar 

Xiao, K. et al. The effect of surface charge on in vivo biodistribution of PEG-oligocholic acid based micellar nanoparticles. Biomaterials 32, 3435–3446 (2011).

Article  CAS  Google Scholar 

Tabata, Y. & Ikada, Y. Effect of the size and surface charge of polymer microspheres on their phagocytosis by macrophage. Biomaterials 9, 356–362 (1988).

Article  CAS  Google Scholar 

Jiang, X., Du, B. & Zheng, J. Glutathione-mediated biotransformation in the liver modulates nanoparticle transport. Nat. Nanotechnol. 14, 874–882 (2019).

Article  CAS  Google Scholar 

Schuh, C. D. et al. Combined structural and functional imaging of the kidney reveals major axial differences in proximal tubule endocytosis. J. Am. Soc. Nephrol. 29, 2696–2712 (2018).

Article  CAS  Google Scholar 

Miller, R. P., Tadagavadi, R. K., Ramesh, G. & Reeves, W. B. Mechanisms of cisplatin nephrotoxicity. Toxins 2, 2490–2518 (2010).

Article  CAS  Google Scholar 

Stamellou, E., Leuchtle, K. & Moeller, M. J. Regenerating tubular epithelial cells of the kidney. Nephrol. Dialysis Transplant. 36, 1968–1975 (2020).

Article  Google Scholar 

Fujigaki, Y. Different modes of renal proximal tubule regeneration in health and disease. World J. Nephrol. 1, 92–99 (2012).

Article  Google Scholar 

Fowler, B. A. Ultrastructural evidence for nephropathy induced by long-term exposure to small amounts of methyl mercury. Science 175, 780–781 (1972).

Article  CAS  Google Scholar 

Melentijevic, I. et al. C. elegans neurons jettison protein aggregates and mitochondria under neurotoxic stress. Nature 542, 367–371 (2017).

Article  CAS  Google Scholar 

Moras, M., Lefevre, S. D. & Ostuni, M. A. From erythroblasts to mature red blood cells: organelle clearance in mammals. Front. Physiol. 8, 1076 (2017).

Article  Google Scholar